Background: Respecting Patients’ privacy is an essential professional responsibility for physicians and other health team members. In this regard, this study investigates medical students’ knowledge and attitude about confidentiality and disclosure of Patients’ information. Methods: In this cross-sectional study, 160 medical students of Iran University of Medical Sciences participated who were selected using stratified random sampling. Data were gathered using a valid and reliable self-report questionnaire. Studentchr('39')s knowledge and attitude toward medical confidentiality were assessed using self-administered and researcher-made questionnaires. Cronbachchr('39')s alpha coefficients for knowledge and attitude levels were 79. 7 and 82. 2, respectively. Results: The average of medical studentschr('39') responses to knowledge and attitude questions were 56. 6% (9. 6/ 17) and 55. 3% (9. 4 out of 17), respectively. On average, females had an acceptable attitude about 57. 5% of the questions, whereas this was 50. 9% for males. On average, females had an acceptable knowledge about 59. 5% of the questions, whereas this was 50. 6% for males. Therefore, female’ s attitudes and knowledge were more correct than their male counterparts (p<0. 001). Conclusion: The low level of knowledge and attitude of medical students towards medical confidentiality indicates that revision of Iranian medical education curriculum to reinforce attention and knowledge of medical students on this issue to render appropriate care to Patients is a necessity. Medical students’ knowledge and attitude towards Patient’ s confidentiality rights is not fulfilling.